Cell Technologies Unit
We can complete any aspect of tissue culture for external companies.
The Cell Technologies Unit manages specialist equipment and laboratories for technologies involving the use and storage of mammalian cells and tissues.
Facilities available through Cell Technologies Unit
Tissue culture and training
Mammalian Cell Propagation: A suite of tissue culture rooms is available for specialist propagation of a diverse range of mammalian cell lines (including stem cells) under normoxic and hypoxic conditions. This facility is capable of culturing cells for external companies; this can be used to test effects of compounds on cells in vitro. The facility can also further investigate the effects the compound is having on the cells to determine a mode of action. It is also possible to train users in the techniques commonly used in tissue culture.
This facility has a two laser eight colour Beckman Coulter Gallios flow cytometer. The cytometer is capable of detecting compounds as small as 0.4mm. This level of detection allows the cytometer to investigate bacteria. Flow cytometry has many varied uses and these routinely include:
- Using fluorescent antibodies to identify and enumerate different cell types
- Immune function
- Investigation of protein levels within different cells types
- Apoptosis and necrosis analysis
- Cell cycle analysis
- Biomarker discovery
- Stem cell analysis
- Pharmaceutical analysis
- Plant cell biology
- Marine biology
To record neuronal activity and neuron reconstruction. This facility is equipped with a specific confocal microscope to aid the analysis of neuronal activity in the slice (in vitro) which is recorded by the patch clamp; and a special cell filling system to label neurons, which are then analysed within our Bioimaging Facility.
Window chamber/skin flap model
Cell and tissue storage
- Thirty -80° freezer units in a temperature controlled environment storing up to 1.2 million samples
- Item tracker labelling and traceability software
- AAW WebReact Temperature Monitoring System
- Cell Radiation: Including Xray sources
- Countess Automated Cell Counter: Easy, accurate cell counting without the need for a hemocytometer
- Specialist Cell Imaging: Leica TCS SP5 Confocal Microscope for multiphoton imaging
- FLEXstation microphysiometer: For measuring transmembrane potentials and intracellular calcium signalling
School of Biomedical Sciences