Self-funded PhD opportunity The production of rhamnolipid biosurfactant by Pseudomonas aeruginosa growing in biofilms.
Subject: Biomedical Sciences
Rhamnolipid biosurfactants produced by Pseudomonas aeruginosa have been investigated as potential replacements for chemical surfactants in a variety of commercial applications. However, P. aeruginosa is a human pathogen and the rhamnolipids also play an important role in pathogenicity including the development of biofilms. Most of the research on production of the rhamnolipids has been carried out using planktonic cultures of the bacterium, but this project will focus on the regulation of the rhamnolipid production when the organism is growing as a biofilm.
The synthesis of rhamnolipid will be investigated in biofilms at various stages of development and production under different growth conditions. There are well established techniques for examining the expression of the relevant genes using qRT-PCR and analytical methods to quantify and characterise the biosurfactant produced all of which have been developed in our laboratory.
Applicants should note that £2000 per annum in bench fees will be required for this project.
M. Dίaz de Rienzo, P. Stevenson, R. Marchant and I.M.Banat (2016). P. aeruginosa biofilm disruption using microbial biosurfactants. Journal of Applied Microbiology, 120: 868—876. DOI: 10.1111/jam.13049
S.K. Satpute, A.G. Banpurkar, I.M. Banat, J.N. Sangshetti, R.R. Patil, W.N. Gade (2016). Multiple Roles of Biosurfactants in Biofilms. Current Pharmaceutical Design 22, 1429-1448.
- Upper Second Class Honours (2:1) Degree or equivalent from a UK institution (or overseas award deemed to be equivalent via UK NARIC)
This is a self-funded PhD opportunity.
The Doctoral College at Ulster University
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