Self-funded PhD opportunity Investigation of the diversity and role of rhamnosyltransferase enzymes in rhamnolipid synthesis in bacteria.

Subject: Biomedical Sciences

Summary

Rhamnolipids have been identified as microbial biosurfactants with huge potential for industrial exploitation, however, the best known producer, Pseudomonas aeruginosa is a human pathogen and therefore unacceptable as an industrial organism. A number of other non-pathogenic bacterial producers of rhamnolipid have been identified and these are currently being intensively researched.  All these bacteria yield a mixture of mono-rhamnolipids and di-rhamnolipids in different proportions since the mono form provides the substrate for a second rhamnosyltransferase to add the second sugar molecule to produce the di form.

It is this second rhamnosyltransferase that will form the focus for this project since it controls the proportion of mono to di-rhamnolipid in the final product. The approach in this project will be to use our existing knowledge of the gene sequences of this enzyme from different organisms to give heterologous expression in E.coli  of a his-tagged protein which can then be purified. Having a purified enzyme will allow protein sequencing to be carried out by mass spectrometry and the development of a cell free enzyme assay to determine the kinetics of each of the rhamnosyltransferases.

Applicants should note that Bench fees of £2000.00 are required.

Related references:

V. U. Irorere, L. Tripathi, R. Marchant, S. McClean and I. M. Banat (2017). Microbial Rhamnolipid Production: A Critical Re-evaluation of Published Data and Suggested Future Publication Criteria. Applied Microbiology & Biotechnology. 101:3941–3951.   DOI 10.1007/s00253-017-8262-0

Dulcey, Carlos Eduardo; de los Santos, Yossef Lopez); Deziel, Eric ; Doucet, Nicolas. (2017). Semi-rational evolution of the Pseudomonas aeruginosa Rhamnosyltransferase 1 subunit A (RhlA) for the synthesis of industrially relevant rhamnolipids. Protein Science,  26:  68-68.

Essential Criteria

  • Upper Second Class Honours (2:1) Degree from a UK institution (or overseas award deemed equivalent via UK NARIC)

Funding

This is a self-funded PhD opportunity.

Other information

The Doctoral College at Ulster University

Launch of the Doctoral College

Current PhD researchers and an alumnus shared their experiences, career development and the social impact of their work at the launch of the Doctoral College at Ulster University.

Watch Video

Key Dates

Submission Deadline
Sunday 1 September 2019
Interview Date
To be confirmed

Contact Supervisor

Professor Ibrahim Banat

Other Supervisors

Apply online

Visit https://www.ulster.ac.uk/applyonline and quote reference number #250387 when applying for this PhD opportunity