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Funded PhD Opportunity

Permanent gene-based therapy: Application of CRISPR/Cas9 targeted nuclease gene therapy to genetic eye disease.

Subject: Biomedical Sciences


Summary

Background to the project:

When the clear window at the front of the eye, known as the cornea, is damaged by injury or disease (including hereditary genetic disease, passed from parents to their children), it can become less transparent causing loss of sight and, at times, blindness. If the cornea becomes too damaged the only treatment available is a corneal transplant from a donor eye. Depending upon which part of the cornea is damaged, all or part of it can be replaced.

However, like all organ donations, there is a shortage of donated eyes. Furthermore, as with any intraocular surgery, there are significant inherent risks, which include the potential of a blinding infection, or subsequent rejection or failure of the graft with time. After 10 years almost half of corneal transplants will have failed meaning that the patient will need another transplant. The risk of rejection increases with subsequent transplants. It is possible to remove a small number of corneal epithelial cells from a patient’s eye and grow sufficient numbers of them in the laboratory to transplant them back onto the eye.

Our laboratory has shown it is possible to correct serious genetic defects within these cells while they are growing in the laboratory, allowing the patient’s own, now healthy normal cells to be placed back on the eye to restore vision.

Methods to be used:

The successful candidate will conduct cutting-edge research in state-of-the art facilities and gain wide experience in a variety of cell and molecular biology techniques. Samples from patients with and without corneal disease and established mouse models of corneal disease are already available. The student will join a group with both national and international collaborations that will allow pursuit of research towards the prevention of this debilitating disease.

Objectives of the Research:

CRISPR/Cas9 is a bacterial enzyme that can be used for genome engineering with unprecedented potential for gene therapy. Its advantage over previous technology is that it is easily reprogrammed, by simply changing a short stretch (20 bases) of RNA sequence, to cut DNA at different positions in the genome, depending on the target one wishes to edit. It can be used to knockout a gene, repair an error or direct the delivery of a therapeutic gene to a specified location.

This project has two aims

1.To test the ability of CRISPR/Cas9 gene editing to prevent the development of corneal disease using, as a model of corneal disease, mice which have been genetically engineered to develop different forms of the human disease.

2.To develop  safe and effective methods and materials to get this treatment to the cells that need it in the eye.

Skills required of applicant:

The applicant should have experience of undertaking a research project, good communication and organisational skills, experience of effective team working and an ability and willingness to learn new skills and techniques, undertake scientific writing and to travel. Applications are welcomed from both medical and non- medical graduates.

References:

https://pure.ulster.ac.uk/en/persons/tara-moore/publications/


Essential criteria

  • Upper Second Class Honours (2:1) Degree or equivalent from a UK institution (or overseas award deemed to be equivalent via UK NARIC)
  • Sound understanding of subject area as evidenced by a comprehensive research proposal

Desirable Criteria

If the University receives a large number of applicants for the project, the following desirable criteria may be applied to shortlist applicants for interview.

  • First Class Honours (1st) Degree
  • Masters at 65%
  • Research project completion within taught Masters degree or MRES
  • Practice-based research experience and/or dissemination
  • Experience using research methods or other approaches relevant to the subject domain
  • Work experience relevant to the proposed project
  • Publications - peer-reviewed
  • Experience of presentation of research findings
  • A comprehensive and articulate personal statement
  • Relevant professional qualification and/or a Degree in a Health or Health related area

Funding

    The University offers the following awards to support PhD study and applications are invited from UK, EU and overseas for the following levels of support:

    Vice Chancellors Research Studentship (VCRS)

    Full award (full-time PhD fees + DfE level of maintenance grant + RTSG for 3 years).

    This scholarship will cover full-time PhD tuition fees and provide the recipient with £15,000 maintenance grant per annum for three years (subject to satisfactory academic performance). This scholarship also comes with £900 per annum for three years as a research training studentship grant (RTSG) allocation to help support the PhD researcher.

    Vice-Chancellor’s Research Bursary (VCRB)

    Part award (full-time PhD fees + 50% DfE level of maintenance grant + RTSG for 3 years).

    This scholarship will cover full-time PhD tuition fees and provide the recipient with £7,500 maintenance grant per annum for three years (subject to satisfactory academic performance). This scholarship also comes with £900 per annum for three years as a research training studentship grant (RTSG) allocation to help support the PhD researcher.

    Vice-Chancellor’s Research Fees Bursary (VCRFB)

    Fees only award (PhD fees + RTSG for 3 years).

    This scholarship will cover full-time PhD tuition fees for three years (subject to satisfactory academic performance). This scholarship also comes with £900 per annum for three years as a research training studentship grant (RTSG) allocation to help support the PhD researcher.

    Department for the Economy (DFE)

    The scholarship will cover tuition fees at the Home rate and a maintenance allowance of £ 15,009 per annum for three years. EU applicants will only be eligible for the fee’s component of the studentship (no maintenance award is provided). For Non-EU nationals the candidate must be "settled" in the UK. This scholarship also comes with £900 per annum for three years as a research training studentship grant (RTSG) allocation to help support the PhD researcher.

    Due consideration should be given to financing your studies; for further information on cost of living etc. please refer to: www.ulster.ac.uk/doctoralcollege/postgraduate-research/fees-and-funding/financing-your-studies


Other information


The Doctoral College at Ulster University


Reviews

My experience has been great and the people that I have worked with have been amazing

Kieran O'Donnell - 3D printing of biological cells for tissue engineering applications

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Completing the MRes provided me with a lot of different skills, particularly in research methods and lab skills.

Michelle Clements Clements - MRes - Life and Health Sciences

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Throughout my PhD I’ve been provided with continuous support and guidance by my supervisors and the staff at the University.I’ve also received many opportunities to further enhance my professional development in the form of teaching experience and presenting my work at conferences which will aid in my pursuit of a career in academia or industry.

William Crowe


Key dates

Submission deadline
Monday 18 February 2019

Interview Date
w/c 11, 18 and 25th March 2019


Applying

Apply Online  


Campus

Coleraine campus

Coleraine campus
Our coastal and riverside campus focussing on science and health


Contact supervisor

Professor Tara Moore


Other supervisors

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